NAD+
INVESTIGATIONALCellular energy and longevity coenzyme
By the INFOPEPTIDOS editorial team · content based on scientific literature.
Updated: August 2026.
Key points
- What it is: essential coenzyme (nicotinamide adenine dinucleotide) present in every cell.
- Main use: cellular energy, longevity, cognition and recovery.
- Typical dose: 50–100 mg subcutaneous (or 250–500 mg IV), in cycles.
- Key result: supports mitochondrial function and activates sirtuins (longevity pathways).
- Common effects: chest tightness or nausea if administered too fast.
- Comparison: energy precursor; complements longevity peptides such as Epitalon.
- Status: not FDA-approved as a treatment (available as a supplement).
Technical data: Molecular formula C21H27N7O14P2 · Molecular weight 663.4 g/mol. Source: PubChem.
Overview
What is NAD+?
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme present in every cell and essential for energy production in the mitochondria. Its levels decline with age, and its replenishment is researched for possible effects on energy, longevity and cognitive function.
Key benefits
Support for cellular energy, DNA repair and the activity of sirtuins, proteins associated with longevity. Many users report more energy, mental clarity and better recovery.
Mechanism of action
NAD+ acts as an electron carrier in energy metabolism and as a substrate for sirtuins and DNA-repair enzymes. Replenishing NAD+ aims to counteract its age-related decline.
Quick start guide
Dosing protocol
| Route | Dose | Frequency | Duration |
|---|---|---|---|
| Subcutaneous | 50–100 mg | Daily or every other day | In cycles |
| Intravenous | 250–500 mg | 1–2 times/week | In cycles |
Administer slowly: fast infusion causes tightness and nausea. The specific dose must be assessed by a healthcare professional.
Reconstitution: units in the syringe
Example with a 500 mg vial + 5 ml of bacteriostatic water → a 100 mg/ml solution. On a U-100 insulin syringe (100 units = 1 ml):
| Dose | Volume | Units (U-100) |
|---|---|---|
| 50 mg | 0.50 ml | 50 U |
| 100 mg | 1.00 ml | 100 U |
Is your vial or water different? Calculate the exact units below.
🧮 Calculate your exact syringe dose →⚠️ Who should NOT use it
- Fast administration: causes chest tightness and nausea — do it slowly.
- Pregnancy or breastfeeding.
- People with serious illness — consult first.
- Always under the supervision of a healthcare professional.
What to expect
- Week 1: often more energy and mental clarity.
- Week 1-2: better recovery and mood.
- Week 2-4: sustained benefits on energy.
- In cycles: repeated according to the longevity goal.
- Side effects: tightness or nausea if administered fast.
- Most effective for: energy, longevity and recovery.
Side effects & safety
- Chest tightness during fast administration.
- Nausea or cramps if applied quickly.
- Transient facial flushing.
- Mild injection-site reaction.
- Discomfort that disappears when slowing down.
- Allergic reaction: rash, itching or swelling.
- Severe chest pain or difficulty breathing.
- Dizziness or fainting.
- Marked palpitations.
- If you are pregnant or planning a pregnancy.
Frequently asked questions
What is NAD+?
It is a coenzyme essential for cellular energy whose levels decline with age; it is researched for longevity effects.
How is NAD+ administered?
Subcutaneously or by slow intravenous route; fast administration causes discomfort.
What is the NAD+ dose?
Around 50-100 mg subcutaneous or 250-500 mg IV, in cycles. The regimen is set by a professional.
What is NAD+ for?
It is researched for energy, longevity, recovery and cognitive function.
What are the side effects?
Mainly chest tightness and nausea when administration is too fast.
Is NAD+ approved?
It is not FDA-approved as a treatment, though it exists as a supplement.
Research areas
- Cellular energy
- Longevity
- Cognitive function
- Recovery
- Mitochondrial health
Related peptides
- Epitalon — Pineal longevity tetrapeptide.
- Glutathione — Master antioxidant; combined in longevity protocols.
- MOTS-c — Mitochondrial peptide for energy and metabolism.
Related articles
Related topics
- NAD+ and longevity
- NAD+ dose
- NAD+ energy
- NAD+ subcutaneous or IV
- NAD+ and sirtuins
- NAD+ side effects
Summary
What it is: Cellular energy and longevity coenzyme.
How it is researched: SC 50-100 mg or IV 250-500 mg, in cycles.
Key features: Supports mitochondria and sirtuins. Not FDA-approved as a treatment.
Related peptides: Epitalon, Glutathione and MOTS-c.
References
- Bhasin S et al. (2023). Nicotinamide Adenine Dinucleotide in Aging Biology: Potential Applications and Many Unknowns. Endocr Rev. PubMed · DOI
- Nadeeshani H et al. (2022). Nicotinamide mononucleotide (NMN) as an anti-aging health product - Promises and safety concerns. J Adv Res. PubMed · DOI
- Kang S et al. (2024). Novel Approach to Skin Anti-Aging: Boosting Pharmacological Effects of Exogenous Nicotinamide Adenine Dinucleotide (NAD(+)) by Synergistic Inhibition of CD38 Expression. Cells. PubMed · DOI
- Hosseini L et al. (2019). Nicotinamide adenine dinucleotide emerges as a therapeutic target in aging and ischemic conditions. Biogerontology. PubMed · DOI
Studies compiled from PubMed for informational purposes; many are preclinical or research-stage and do not constitute medical advice.